Studies of the role and function of barley stripe mosaic virus encoded proteins in replication and movement using GFP fusions.
Identifieur interne : 000635 ( Main/Exploration ); précédent : 000634; suivant : 000636Studies of the role and function of barley stripe mosaic virus encoded proteins in replication and movement using GFP fusions.
Auteurs : Sophie Haupt [Royaume-Uni] ; Angelika Ziegler ; Graham Cowan ; Lesley TorranceSource :
- Methods in molecular biology (Clifton, N.J.) [ 1064-3745 ] ; 2009.
Descripteurs français
- KwdFr :
- ADN complémentaire (génétique), ADN complémentaire (métabolisme), ARN viral (génétique), ARN viral (isolement et purification), ARN viral (métabolisme), Chloroplastes (métabolisme), Expression des gènes (MeSH), Feuilles de plante (virologie), Génome viral (génétique), Phénomènes physiologiques viraux (MeSH), Protéines de fusion recombinantes (génétique), Protéines de fusion recombinantes (métabolisme), Protéines virales (génétique), Protéines virales (métabolisme), Protéines à fluorescence verte (analyse), Protéines à fluorescence verte (génétique), Protéines à fluorescence verte (métabolisme), Rhizobium (génétique), Rhizobium (métabolisme), Tabac (virologie), Transgènes (génétique), Virus à ARN (génétique), Virus à ARN (métabolisme).
- MESH :
- analyse : Protéines à fluorescence verte.
- génétique : ADN complémentaire, ARN viral, Génome viral, Protéines de fusion recombinantes, Protéines virales, Protéines à fluorescence verte, Rhizobium, Transgènes, Virus à ARN.
- isolement et purification : ARN viral.
- métabolisme : ADN complémentaire, ARN viral, Chloroplastes, Protéines de fusion recombinantes, Protéines virales, Protéines à fluorescence verte, Rhizobium, Virus à ARN.
- virologie : Feuilles de plante, Tabac.
- Expression des gènes, Phénomènes physiologiques viraux.
English descriptors
- KwdEn :
- Chloroplasts (metabolism), DNA, Complementary (genetics), DNA, Complementary (metabolism), Gene Expression (MeSH), Genome, Viral (genetics), Green Fluorescent Proteins (analysis), Green Fluorescent Proteins (genetics), Green Fluorescent Proteins (metabolism), Plant Leaves (virology), RNA Viruses (genetics), RNA Viruses (metabolism), RNA, Viral (genetics), RNA, Viral (isolation & purification), RNA, Viral (metabolism), Recombinant Fusion Proteins (genetics), Recombinant Fusion Proteins (metabolism), Rhizobium (genetics), Rhizobium (metabolism), Tobacco (virology), Transgenes (genetics), Viral Proteins (genetics), Viral Proteins (metabolism), Virus Physiological Phenomena (MeSH).
- MESH :
- chemical , analysis : Green Fluorescent Proteins.
- chemical , genetics : DNA, Complementary, Green Fluorescent Proteins, RNA, Viral, Recombinant Fusion Proteins, Viral Proteins.
- genetics : Genome, Viral, RNA Viruses, Rhizobium, Transgenes.
- chemical , isolation & purification : RNA, Viral.
- metabolism : Chloroplasts, DNA, Complementary, Green Fluorescent Proteins, RNA Viruses, RNA, Viral, Recombinant Fusion Proteins, Rhizobium, Viral Proteins.
- virology : Plant Leaves, Tobacco.
- Gene Expression, Virus Physiological Phenomena.
Abstract
This chapter describes techniques to investigate the localisation and function of virus-encoded proteins in plants using green fluorescent protein (GFP) transiently expressed from plasmids or infectious cDNA reporter clones of barley stripe mosaic virus. Virus movement and the localisation of GFP-tagged proteins in living cells were monitored by confocal laser scanning microscopy (CLSM). In addition, GFP expression was imaged in transgenic plants where specific organelles or subcellular structures such as endoplasmic reticulum were labelled with another fluorophore (e.g., monomeric red fluorescent protein). Using these approaches we discovered evidence for additional roles played by virus encoded movement protein TGB2 and gammab protein in virus replication. Methods are described for clone construction and mutagenesis, and for transient expression (biolistic bombardment or agrobacterium infiltration) in the epidermal cells of Nicotiana benthamiana or barley. In addition, techniques for chloroplast isolation and imaging of the different fluorescent proteins, and the avoidance of interference from autofluorescence, are described.
DOI: 10.1007/978-1-59745-559-6_20
PubMed: 19378124
Affiliations:
Links toward previous steps (curation, corpus...)
Le document en format XML
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Virus movement and the localisation of GFP-tagged proteins in living cells were monitored by confocal laser scanning microscopy (CLSM). In addition, GFP expression was imaged in transgenic plants where specific organelles or subcellular structures such as endoplasmic reticulum were labelled with another fluorophore (e.g., monomeric red fluorescent protein). Using these approaches we discovered evidence for additional roles played by virus encoded movement protein TGB2 and gammab protein in virus replication. Methods are described for clone construction and mutagenesis, and for transient expression (biolistic bombardment or agrobacterium infiltration) in the epidermal cells of Nicotiana benthamiana or barley. In addition, techniques for chloroplast isolation and imaging of the different fluorescent proteins, and the avoidance of interference from autofluorescence, are described.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">19378124</PMID><DateCompleted><Year>2009</Year><Month>05</Month><Day>19</Day></DateCompleted><DateRevised><Year>2016</Year><Month>11</Month><Day>25</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Print">1064-3745</ISSN><JournalIssue CitedMedium="Print"><Volume>515</Volume><PubDate><Year>2009</Year></PubDate></JournalIssue><Title>Methods in molecular biology (Clifton, N.J.)</Title><ISOAbbreviation>Methods Mol Biol</ISOAbbreviation></Journal><ArticleTitle>Studies of the role and function of barley stripe mosaic virus encoded proteins in replication and movement using GFP fusions.</ArticleTitle><Pagination><MedlinePgn>287-97</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1007/978-1-59745-559-6_20</ELocationID><Abstract><AbstractText>This chapter describes techniques to investigate the localisation and function of virus-encoded proteins in plants using green fluorescent protein (GFP) transiently expressed from plasmids or infectious cDNA reporter clones of barley stripe mosaic virus. Virus movement and the localisation of GFP-tagged proteins in living cells were monitored by confocal laser scanning microscopy (CLSM). In addition, GFP expression was imaged in transgenic plants where specific organelles or subcellular structures such as endoplasmic reticulum were labelled with another fluorophore (e.g., monomeric red fluorescent protein). Using these approaches we discovered evidence for additional roles played by virus encoded movement protein TGB2 and gammab protein in virus replication. Methods are described for clone construction and mutagenesis, and for transient expression (biolistic bombardment or agrobacterium infiltration) in the epidermal cells of Nicotiana benthamiana or barley. In addition, techniques for chloroplast isolation and imaging of the different fluorescent proteins, and the avoidance of interference from autofluorescence, are described.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Haupt</LastName><ForeName>Sophie</ForeName><Initials>S</Initials><AffiliationInfo><Affiliation>Scottish Crop Research Institute, University of Dundee, Dundee, UK.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Ziegler</LastName><ForeName>Angelika</ForeName><Initials>A</Initials></Author><Author ValidYN="Y"><LastName>Cowan</LastName><ForeName>Graham</ForeName><Initials>G</Initials></Author><Author ValidYN="Y"><LastName>Torrance</LastName><ForeName>Lesley</ForeName><Initials>L</Initials></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList></Article><MedlineJournalInfo><Country>United States</Country><MedlineTA>Methods Mol Biol</MedlineTA><NlmUniqueID>9214969</NlmUniqueID><ISSNLinking>1064-3745</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D018076">DNA, Complementary</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D012367">RNA, Viral</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D011993">Recombinant Fusion Proteins</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D014764">Viral Proteins</NameOfSubstance></Chemical><Chemical><RegistryNumber>147336-22-9</RegistryNumber><NameOfSubstance UI="D049452">Green Fluorescent Proteins</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D002736" MajorTopicYN="N">Chloroplasts</DescriptorName><QualifierName UI="Q000378" 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Leaves</DescriptorName><QualifierName UI="Q000821" MajorTopicYN="N">virology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D012328" MajorTopicYN="N">RNA Viruses</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D012367" MajorTopicYN="N">RNA, Viral</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000302" MajorTopicYN="N">isolation & purification</QualifierName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D011993" MajorTopicYN="N">Recombinant Fusion Proteins</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D012231" MajorTopicYN="N">Rhizobium</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D014026" MajorTopicYN="N">Tobacco</DescriptorName><QualifierName UI="Q000821" MajorTopicYN="N">virology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D019076" MajorTopicYN="N">Transgenes</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D014764" MajorTopicYN="N">Viral Proteins</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D018406" MajorTopicYN="Y">Virus Physiological Phenomena</DescriptorName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="entrez"><Year>2009</Year><Month>4</Month><Day>21</Day><Hour>9</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2009</Year><Month>4</Month><Day>21</Day><Hour>9</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2009</Year><Month>5</Month><Day>20</Day><Hour>9</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">19378124</ArticleId><ArticleId IdType="doi">10.1007/978-1-59745-559-6_20</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>Royaume-Uni</li></country></list><tree><noCountry><name sortKey="Cowan, Graham" sort="Cowan, Graham" uniqKey="Cowan G" first="Graham" last="Cowan">Graham Cowan</name><name sortKey="Torrance, Lesley" sort="Torrance, Lesley" uniqKey="Torrance L" first="Lesley" last="Torrance">Lesley Torrance</name><name sortKey="Ziegler, Angelika" sort="Ziegler, Angelika" uniqKey="Ziegler A" first="Angelika" last="Ziegler">Angelika Ziegler</name></noCountry><country name="Royaume-Uni"><noRegion><name sortKey="Haupt, Sophie" sort="Haupt, Sophie" uniqKey="Haupt S" first="Sophie" last="Haupt">Sophie Haupt</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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